Figure 6.

AID interacts with the H2B N-tail. (A) EMSAs show that the AID lengths of hMid–AID are critical for complex formation with DSB nucleosomes and the simultaneous increase in hexasome production. Experiments were repeated at least twice. (B) GF analyses show that AID and the H2B N-tail are required for interaction between hMid–AID and H2A–H2B. Protein mixtures with stoichiometric amounts were eluted from a Superdex200 10/300 column in 500 mM NaCl. Elution profiles and the corresponding SDS-PAGE are shown at the left and right sides, respectively.