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. Author manuscript; available in PMC: 2016 Jul 15.
Published in final edited form as: Neuron. 2015 Jul 15;87(2):371–381. doi: 10.1016/j.neuron.2015.06.034

Figure 3. Phosphorylated Ubiquitin at S65 Is Increased during Mitochondrial Dysfunction in the CNS.

Figure 3

(A) UB-AQUA proteomics of total UB, individual UB chain linkages, and phospho-S65 UB in whole-cell cortical lysates. Ubiquitin chain linkage key: blue, M1 linear chains; lime, K6 linkages; gold, K11 chain linkages; red, K27 chain linkages; pink, K29 chain linkages (not quantifiable); violet, K33 chain linkages; navy, K48 chain linkages; aqua, K63 chain linkages; green, p-S65 UB. n = 5/group.

(B) Percentage of phospho-S65 UB normalized to the total percentage of UB in cortical samples. n = 5/group.

(C) UB-AQUA proteomics of total UB, individual UB chain linkages, and phospho-S65 UB in whole-cell liver lysates. Ubiquitin chain linkage key: blue, M1 linear chains; lime, K6 linkages; gold, K11 chain linkages; red, K27 chain linkages; pink, K29 chain linkages (not quantifiable); violet, K33 chain linkages; navy, K48 chain linkages; aqua, K63 chain linkages; green, p-S65 UB. n = 5/group.

(D) Percentage of phospho-S65 UB normalized to the total percentage of UB in liver samples. n = 5/group. (A–D) **p < 0.01; ***p < 0.001; NS, not significant. Mean ± SEM. ANOVA Tukey's post hoc test.