Figure 6.

Phenotypic analysis of Hfe^−/− Hjv^−/− mice. Analysis of (A) serum transferrin (Tf) saturation (%), (B) non-heme liver iron and (C) spleen iron (μg/g wet weight). WT (n=7), Hfe^−/− (n=8), Hjv^−/− (n=8) and Hfe^−/− Hjv^−/− (n=10) animals are depicted. Total mRNA was harvested from livers (n=6 for each genotype) and hepcidin (Hamp) mRNA (D) was assessed by quantitative real-time PCR, and normalized to β-actin (Actb). Serum Hepc-1 (E) was measured by murine immunoassay and plotted against (F) relative hepcidin mRNA measured by quantitative real-time PCR. Serum and quantitative real-time PCR measured hepcidin-1 levels were compared to serum transferrin saturation (G,%) and non-heme liver iron (H, μg/g wet weight). Ratios are expressed ± SEM. P values were calculated using the Student t-test ****P<0.001, ***P<0.005, **P<0.01 and *P<0.05.