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Effects of PLX4032, SAHA, and TSH on thyroid gene expression in thyroid cancer cells. The upper four thyroid cancer cell lines harbored BRAF V600E and the lower four harbored the wild-type BRAF. Cells were treated with 0.5 μM PLX4032 and/or 2 μM SAHA for 72 hours, with/without 20 mU/mL TSH (black bar) added at the 24-hour time point and continued for 48 hours. Data are presented as the mean ± SD of values from three assays. *, P < .05, **, P < .01 for comparison with control.
