Figure 4.

Immunofluorescent microscopic analysis of NIS protein expression in SW1736 cells. Cells were treated with control DMSO (Con), PLX4032 (P), SAHA (S), and TSH (T) as indicated and with the conditions and concentrations described in the legend to Figure 2. Double-immunofluorescent microscopy was displayed, with the blue color representing 4′,6-diamidino-2-phenylindole nuclear staining and the green color representing NIS protein expression. The abundant NIS expression in cells treated with the indicated agents was in striking contrast with the control cells, which showed only minimal expression. The most robust expression of NIS was seen with the triple treatment with PLX4032, SAHA, and TSH, in which cell membrane localization of NIS could be appreciated (represented by the increased peripheral staining of green color of the cell). Scale bar, 50 μm.