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. 2016 Mar 22;11(3):e0152193. doi: 10.1371/journal.pone.0152193

Fig 1. Bacterial growth and CXCL8 release in HEp2 cells.

Fig 1

A) Fluorescent micrographs demonstrating the infectivity of W. chondrophila at an apparent MOI of 10 at 24h post-infection. W. chondrophila inclusions are labelled green using an anti-Waddlia rabbit polyclonal antisera and FITC anti-rabbit secondary antibody, host cell nuclei are stained in blue (DAPI). The scale bars correspond to 100μm. B) W. chondrophila infection induces CXCL8 production at 24 hours pi in HEp2 cells. Cells were to medium containing uninfected cell lysate (Med), W. chondrophila (Live) or UV-killed organisms (MOI 10) for 24h. Statistically-significant differences relative to medium control are indicated by ***P<0.001. Data were analysed by one way ANOVA followed by Fishers LSD test.