Fig 6.
Glycine reduces VC L5PyNs dendritic current spread A: Membrane time constants Ƭ0 and Ƭ1. were calculated by plotting the natural log of the response expressed as percentage of the peak negative potential (% ΔEmax) to “peel” the first order exponential for time points lying between 5 and 15ms. Ƭ0 could then be read as the slope negative inverse of the regression line. The second order exponential for time points earlier than 5ms was peeled by plotting the difference between the Ƭ0 regression line from the points lying above this line and normalizing the y-intercept of the Ƭ1 regression line to 100%. Ƭ1 could then be read as the slope negative inverse of the normalized Ƭ1 regression line. B: Calculation of the electrotonic length (L) using the equation L = π (Ƭ0/ Ƭ 1)-1/2 showed that L is significantly increased in the presence of 100μM glycine, indicating that the dentritic current spread attenuation is higher in the latter case. **p<0.01,***p<0.001.