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. 2016 Mar 22;12(3):e1005514. doi: 10.1371/journal.ppat.1005514

Fig 9. Incorporation of mRNAs into granules after severe heat shock.

Fig 9

A. Analysis of RNA samples taken during the granules enrichment. Equal volume of the RNA samples (except SN1) isolated from untreated and heat-shocked procyclic cells were probed for total mRNA, ZC3H11, HSP70 and β-tubulin mRNAs. For SN1 samples only half of the volume was loaded to prevent gel overloading. ‘T’ is RNA sample prepared in parallel as a control for RNA quality and is not equivalent to the other samples. One representative experiment out of three separate repeats is shown. B. The percentage of the total mRNA, ZC3H11, HSP70 and β-tubulin mRNAs (mean ± standard deviation for three independent experiments) quantified from Northern blots (A). This percentage is calculated based on total mRNA signal being the sum of SN1, SN2, SN3, SN4 and G.