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. 2016 Mar 22;12(3):e1005514. doi: 10.1371/journal.ppat.1005514

Fig 10. mRNAs with ZC3H11 3'-UTRs are distributed throughout the cytosol.

Fig 10

Single mRNA FISH was done using Affymetrix probes. Procyclic cells expressing the CAT reporter with full-length ZC3H11 3'-UTR (A) or fragment 1 of the ZC3H11 3'-UTR (B) were heat-shocked for 1 hour at 41°C, or left at 27°C. Slides were prepared and hybridized with a probe sets antisense to the CAT ORF (red) and the spliced leader (SL) probe (green) together with DAPI staining (blue). One representative image of untreated and heat-shocked cells out of several experiments is shown.