Table 8. Analysis of energy metabolism in line Pou3f3L423P.
| Males | Females | Genotype: P-value | ||||
|---|---|---|---|---|---|---|
| Test | Parameter | Homozygous mutant | Wild-type | Homozygous mutant | Wild-type | |
| Indirect calorimetry | Body weight (g) | 24.3 ± 1.3 | 30.7 ± 2.5 | 20.9 ± 2.1 | 28.4 ± 3.3 | c |
| Body temperature (°C) | 35.7 ± 0.7 | 36.2 ± 0.4 | 36.4 ± 0.3 | 36.8 ± 0.3 | b | |
| Food intake (g) | 2.8 ± 0.3 | 3.2 ± 0.8 | 2.8 ± 0.4 | 3.6 ± 0.6 | ||
| Average VO2 consumption (ml/h animal) | 85 ± 4 | 95 ± 6 | 81 ± 6 | 99 ± 7 | a | |
| Average RER (VCO2/VO2) | 0.89 ± 0.01 | 0.90 ± 0.04 | 0.90 ± 0.02 | 0.92 ± 0.03 | ||
| Adjusted mean MR (mW) | 507 ± 21 | 508 ± 21 | 515 ± 23 | 556 ± 50 | 0.05 | |
| Adjusted RMR (mW) | 375 ± 17 | 383 ± 23 | 392 ± 11 | 492 ± 24 | c | |
| Average distance (cm/20 min) | 843 ± 213 | 658 ± 86 | 958 ± 400 | 810 ± 298 | 0.06 | |
| Average rearings (counts/20 min) | 144 ± 59 | 119 ± 20 | 144 ± 71 | 149 ± 69 | ||
| TD-NMR | Body weight (g) | 24.8 ± 1.6 | 30.5 ± 2.6 | 20.1 ± 2.8 | 27.4 ± 3.2 | c |
| Fat mass (g) | 5.4 ± 0.6 | 7.8 ± 1.4 | 4.9 ± 1.1 | 8.0 ± 1.9 | ||
| Lean mass (g) | 15.8 ± 1 | 18.9 ± 1.3 | 12.2 ± 1.5 | 16.1 ± 1.5 | 0.06 |
RER, respiratory exchange ratio; adjusted mean MR, metabolic rate (MR) was adjusted for body mass by subtracting residual MR from modeled mean expected MR; adjusted RMR, adjusted resting metabolic rate (i.e. lowest MR during the measurement); TD-NMR, time domain nuclear magnetic resonance.
Analysis was done on 11-week-old mice under ad libitum conditions. No. per genotype and sex: n = 9–16. Substrate utilization rates during indirect calorimetry were calculated as described [27]. Data are presented as mean ± standard deviation. Significance vs. wild-type controls: a, P < 0.05; b, P < 0.01; c, P < 0.001.