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. 2016 Mar 22;11(3):e0149394. doi: 10.1371/journal.pone.0149394

Fig 2. The suppressive effect of 3-HBA on rat VSMC migration.

Fig 2

(A, B) VSMCs were serum starved for 24 h and then pretreated with 3-HBA (0, 100 μM) for 24 h. VSMCs were stimulated with PDGF (A,B) 25 ng/ml for 24 h. (A) Before stimulation, wells were scratched, and cells were stained with hematoxylin and eosin and scored using Image J. (B) MMP-2 protein levels were determined by Western blot analysis. ** indicates p < 0.005 compared to the PDGF group. Values represent the mean ± SEM of three independent sets of experiments.