FIGURE 3:

Increased global transcription is associated with increased H3K9ac labeling intensity in myonuclei. H3K9ac staining within myonuclei was quantified on muscle cross-sections at various time points after mechanical overload. Arrows indicate H3K9ac-positive myonuclei. (A) Representative images for H3K9ac staining in skeletal muscle cross-sections. Scale bar, 50 μm. (B) Quantification of the percentage of myonuclei that stained positive for H3K9ac during mechanical overload. There was no significant change in the percentage of H3K9ac staining (p < 0.05 and n = 3 or 4 mice per time point). (C) Quantification of the integrated density of H3K9ac labeling per myonucleus during mechanical overload. *Significantly higher integrated density of H3K9ac labeling at the SA3 and SA7 time points relative to Sham (p < 0.05 and n = 3 or 4 mice per time point). Data are presented as mean ± SEM.