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. 2016 Mar 1;27(5):838–847. doi: 10.1091/mbc.E15-10-0707

FIGURE 2:

FIGURE 2:

CNK4 is a flagellar protein. (A) CNK4 is localized to flagella and basal body. wt and cnk4::CNK4-HA cells were immunostained with anti-HA and anti–α-tubulin antibodies, respectively. High-exposure images show the flagellar location of CNK4. Bar, 10 μm. (B) cnk4::CNK4-HA cells were fractionated into cell body and flagella, followed by immunoblotting. Here 1×Fla (flagella) represents equal proportion of flagella to the cell body. (C) Isolated flagella of cnk4::CNK-HA cells were fractionated into membrane matrix (M+M) and axonemal fractions, followed by immunoblotting. FMG1 is a membrane protein. (D) wt cells were allowed to regenerate flagella after deflagellation (left) or treated with sodium pyrophosphate (NaPPi) to induce flagellar disassembly (right), followed by immunoblotting. (E) Flagella were isolated during flagellar regeneration after deflagellation or flagellar disassembly induced by NaPPi, followed by immunoblotting. Con, steady-state flagella; Dis, disassembling flagella; Reg, regenerating flagella.