Fig. 4.

Excerpts from Clustal Omega and KMAD alignments of human sialoprotein (SIAL_HUMAN) with four homologues. (A) Clustal Omega alignment annotated with PTMs; (B) KMAD alignment annotated with PTMs. PTM sites are highlighted with bright colours (red is annotated phosphorylation, orange is predicted phosphorylation, green is N-linked glycosylation; further colour details are given on the project website). The two serines that seem to appear in the sequences 4 and 5 around position 93 in the KMAD alignment are located in the gap (indicated with lowercase characters) around position 90 in the Clustal Omega alignment. In DNA analysis, protein engineering, etc, this software feature is used frequently. The gapped alignment that provides a more phylogeny-oriented view on this sequence family is available at the KMAD website