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. Author manuscript; available in PMC: 2016 Mar 22.
Published in final edited form as: Neurosci Biobehav Rev. 2010 Dec 21;35(9):1847–1853. doi: 10.1016/j.neubiorev.2010.12.008

Fig. 1.

Fig. 1

Overhead view of the arena for CPP experiments which contained four quadrants of two different visual areas, arranged diagonally from each other. The arena was lit from below with distinct, alternating black/white stripes in one area. Free-ware, Java-based software was used to track the crayfish in a video feed from a camera mounted above the tank. In a conditioning session a crayfish was isolated in a specific quadrant (A) with a removable Plexiglas enclosure for 30 min where amphetamine or cocaine was infused for the first 5 min. When allowed to move freely about the entire arena (B) following 5 successive days of psychostimulant conditioning, crayfish exhibited a strong preference for the environment that had been paired with amphetamine or cocaine.