Real time whole-cell respiration was obtained in each of the three differentiation fates, namely adipogenesis (A), chondrogenesis (B) and osteogenesis (C). Oligomycin, CCCP and rotenone plus antymycin were added where indicated. Basal oxygen consumption rates (D), ATP-production dependent OCR (E), Maximal (F), Spare respiratory capacity (G), H+ Leak-linked OCR (H) and Non-mitochondrial respiration (I) were calculated from data such as those shown in panels A–C. * p ≤ 0.05, ** p ≤ 0.001, ns = not significant.