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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Stem Cells. 2015 Dec 21;34(3):743–755. doi: 10.1002/stem.2248

Figure 7. MSC differentiation is dependent on mitochondrial fusion and fission.

Figure 7

MSCs were transduced with adenovirus harboring siRNA Mfn2 or carrying a superexpression vector for a dominant negative form of drp1. Adipogenic differentiation proficiency of mfn2Scr versus mfn2Si was evaluated through Oil Red O lipid storage vacuole histochemistry (A) and validated through qPCR for specific markers (B) PPARγ (B) CREB and (C) RXR. Chondrogenic differentiation proficiency of drp1KD cells was evaluated in comparison to drp1Scr cells through the presence of proteoglycan rich ECM stained with toluidine blue (E) and validated through qPCR for specific markers (F) Col2a, N-cadherin (Ncad) (G) and Sox9 (H). Osteogenic differentiation proficiency of mfn2Scr versus mfn2Si was evaluated through Alizarin Red calcified ECM histochemistry (I) and validated through qPCR for specific markers (J) Runx2 (K) Osterix (Osx) and (L) Dlx-5. * p ≤ 0.05, ** p ≤ 0.001, ns = not significant. Bar = 100 μm.