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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Genesis. 2016 Feb 5;54(3):129–135. doi: 10.1002/dvg.22918

Figure 1.

Figure 1

Nrl-Cre is expressed specifically in rods. The 1.7 kb Nrl promoter upstream of Nrl exons 1 (E1) and 2 (E2) with a 724bp intron was used to drive Cre expression (a). tdTomato fluorescence (red) in Nrl-Cre; Ai9 (b,e) and Cre negative, Ai9 (c,f) frozen retinal cross-sections at 1 month of age are shown. Immunostaining of 1 month old Nrl-Cre; Ai9 retinas with Cone Arrestin (CAR, green) shows that tdTomato is not expressed in CAR+ cones (yellow arrows in d,g,i). Reverse Transcriptase-PCR (RT-PCR) analysis of Cre expression in total RNA from the indicated tissues of 1 month old Nrl-Cre mice (h). β-Actin served as a control for RNA quality. A sample without reverse transcriptase (Retina -RT) serves as a negative control. Cre expression was only detected in the retina. ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer; OS, outer segment; IS, inner segment.