Fig. 4. Chemical derivatization for fatty acids. (A) Methyl esterification reaction of fatty acids. (B) pentafluorobenzyl (PFB) derivatization reaction for short-chain fatty acids. (C) Trimethylsilyl derivatization reaction for short-chain fatty acids. PFBB, 2,3,4,5,6-pentafluorobenzyl bromide. BSTFA, N, O-bis(trimethyl-silyl) trifluoroacetamide.
