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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: Psychopharmacology (Berl). 2016 Feb 9;233(7):1299–1310. doi: 10.1007/s00213-016-4217-x

Figure 1.

Figure 1

Schematic representation of stress testing protocol: aCardiovascular measures [heart rate (HR), systolic blood pressure (SBP), and diastolic blood pressure (DBP)] were collected (1) every two minutes during the last 10-min of the venipuncture recovery period; (2) at the beginning of the speech preparation period; (3) at the beginning and at minute two of the speech task; (4) every two minutes of the PASAT task; and (5) every five minutes of the first 20 minutes of the post-stress recovery period. These measures were then averaged to obtain a mean speech preparation, speech, and PASAT task value for each cardiovascular measure. bBlood for cortisol was collected at the end of venipuncture recovery and again 10, 20, 30, and 40 minutes post-stressor completion. cBlood for adrenocorticotrophic hormone (ACTH) was collected (1) at the end of venipuncture recovery; (2) immediately following stressor completion (after the PASAT task); and (3) 10 and 20 minutes post-stressor completion. Blood for dprogesterone (PROG) and neurosteroids [ALLO, Pregnanolone, 3α,5β-THDOC (THDOC)] was collected (1) at the end of venipuncture recovery period; (2) minute two of the speech task; (3) immediately following stressor completion (after the PASAT task); and (4) 10, 20, 30, and 40 minutes post-stressor completion.