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. Author manuscript; available in PMC: 2017 Apr 7.
Published in final edited form as: Vaccine. 2015 Dec 22;34(16):1945–1955. doi: 10.1016/j.vaccine.2015.12.038

Figure 1.

Figure 1

Gating strategies for B cells. Blood samples were tested for surface or intracellular staining by flow cytometry. PBMCs were tested for annexin V binding. (A) Representative dot plots, displaying the gating strategy used to assess the parameters of B cells. Live PBMCs were gated based on forward scatter (FSC) and side scatter (SSC). Next, total B cells were gated on CD19+ cells. B cell apoptosis and cycling expression were analyzed by percentages of annexin V binding and ki67+ B cells. (B) The median frequencies of annexin V binding B cells from controls (circles) and patients (stars) on D0 and D7 post-vaccination. (C) The median frequencies of ki67+ B cells from controls (circles) and patients (stars) on D0 and D7 post-vaccination.