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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: J Mol Med (Berl). 2016 Feb 2;94(3):277–286. doi: 10.1007/s00109-016-1386-3

Fig. 3.

Fig. 3

IL10 gene is regulated by HIF-1α. a HIF-1α protein was stabilized by deferoxamine (DFO) in mouse CD4+ T cells in a dose-dependent manner and IL-10 mRNA levels quantified by real-time RT-PCR (n=3). b Hypoxia (5% O2) induced HIF-1α protein was knocked down in mouse CD4+ T cells using shRNA and IL-10 mRNA levels were measured. Nonspecific shRNA (NS) was used as control (n=3). c Hypoxia (5% O2) induced HIF-1α protein was knocked down in the Raw 264.7 macrophage cells using shRNA and IL-10 mRNA levels were measured. Nonspecific shRNA (NS) was used as control (n=3). d The sites of candidate hypoxia response elements (HRE, consensus sequence Pu(A/G)CGTG) in the promoter and intron 4 sequences of the mouse IL10 gene are numbered in shaded boxes. HIF-1α interaction with the putative HRE sites was examined by chromatin immunoprecipitation and quantified using real-time PCR in mouse CD4+ cells cultured in a 5% O2 incubator. Values shown as mean ± SEM, *p < 0.05.