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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: J Invest Dermatol. 2016 Jan;136(1):182–191. doi: 10.1038/JID.2015.366

Figure 3.

Figure 3

Ectopic expression of miR-203 can restore the galectin-7 knockdown phenotype of keratin-1 expression. (a) The proliferated miR-203 knockdown clones were monitored using time-lapse microscopy. Scale bar = 50μm. (b) The proliferation rate of miR-203 knockdown clones was quantified by counting the number of progeny cells 24 h after the first round of division (n = 20). (c – h) HaCaT cells were transiently transfected with indicated plasmids and their corresponding control plasmids. (c–f) Real-time PCR; (g and h) Immunoblot analysis are presented. All experiments were performed at least in triplicate, and the statistical analyses were performed by individually comparing with HaCaT parental cells (P), ns: not significant, ***P < 0.001.