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. 2016 Mar 23;6:34. doi: 10.3389/fcimb.2016.00034

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Copyright © 2016 Graillot, Dormoy, Dupuy, Shay, Huc, Mirey and Vignard.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Chronic exposure to EcolCDT enhances anchorage-independent growth in 1CTA and 1CTP but not in 1CT and 1CTR. (A) HCECs and HCECs^CE were cultured in soft-agar in 6-well plates for 3 weeks in triplicates, and colonies larger than 100 μm in size were scored. (B) Quantification of the soft-agar assay presented in (A). Results represent the mean ± SEM of three independent experiments; statistical differences were analyzed by a Student's t-test (indicated by asterisks) between the indicated conditions, or by one-way ANOVA (indicated by dollars) for multiple comparisons, followed by Tukey's HSD post-hoc test (** or $$ P < 0.01; $$$ P < 0.001).