Skip to main content
. 2016 Mar 23;7:326. doi: 10.3389/fmicb.2016.00326

Figure 1.

Figure 1

DVGs accumulate in viral stocks when expanded at high multiplicity of infection (MOI) or extended periods of time. As examples to illustrate the accumulation of DVGs in virus stocks propagated in different conditions (A) shows viral PCR products amplified from A549 cells infected with a MOI of 2, 0.2, or 0.02 of IAV HK/x31 (X31) (H3N2) for 6 h. (B) Shows viral PCR products amplified from A549 cells infected with a MOI 0.1 of A/California/7/2009 (CA) (H1N1) for 2, 6, 12, 24, or 48 h. Position of base pair size reference markers is indicated in each gel. Asterisks indicate DVGs confirmed by sequencing.