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. 2016 Mar 23;7:326. doi: 10.3389/fmicb.2016.00326

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Copyright © 2016 Xue, Chambers, Hensley and López.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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IAV DVGs are found in live-attenuated influenza vaccines. (A) PCR products detecting DVGs in live attenuated influenza vaccine stocks. Lanes 1, 2, and 3 represent live influenza vaccine NR-17600 intranasal spray formula for the 2009–2010 season, live influenza vaccine NR-21987 intranasal spray formula for the 2010-2011 season, and live influenza vaccine NR-36465 intranasal spray formula for the 2011-2012 season, respectively. (B) PCR products identifying DVGs in A549 cells infected with the different vaccine stocks. Vaccine isolates were obtained from BEI Bioresources. Position of base pair size reference markers is indicated in each gel. Asterisks indicate DVGs confirmed by sequencing.