Figure 6.

Example of the generation of a IAV virus stock free of DVGs. (A) Viral PCR products amplified from A549 cells infected with a MOI 0.1 of a parental stock of A/New Caledonia /03/2005 at different times post infection. (B) Viral PCR products amplified from MDCK cells infected for 48 h with A/New Caledonia /03/2005 seed virus prepared after plaque purification at 10⁻⁵ dilution (around 300 TCID₅₀ per well in 6-well plate) from the original stock. (C) Viral PCR products amplified from A549 cells infected with different MOIs of working stocks of A/New Caledonia /03/2005 containing a high load of DVGs (HD) or depleted of DVGs (LD) following the proposed protocol. Position of base pair size reference markers is indicated in each gel. Asterisks indicate DVGs confirmed by sequencing.