| Reagent | Volume μl |
|---|---|
| 2X Reaction Mix (Invitrogen #12574-018) | 12.5 |
| MB Tuni 12 (or specific F primer) | 0.5 |
| MB Tuni 13 (or specific R primer) | 0.5 |
| SS III RT/Platinum Taq mix | 1.0 |
| RNA | 1 μg |
| dH2O | to 25 μl total |
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