Fig. 3.

Confirmation by Sanger sequencing of the somatic point substitution in the tumor sample from a 37 y/o patient diagnosed with adenocarcinoma. This mutation represents the third most frequently mutated codon in the TP53 gene. The mutation in the tumor sample (B) is a heterozygous substitution which occurs at a frequency of 15.96% (highlighted in purple). Despite this rather low cellularity, the total absence of background noise allows for an easy detection of the green A signal in the Sanger sequencing track, which is instead clearly absent in the healthy tissue (A).