Table 1.

Relevant bioprocess parameters for different mAb expression systems

Cell line	Peak cell concentration	Specific growth rate	Specific productivity	Yield	STY	Process type	Reference
	[106 cells/mL]	[per day]	[pg/cell/day]	[g/L]	[mg/(L × day)]
CHO	8.5	0.7	>50	0.9	121	Batch	Reinhart et al. (2015)
	23.9	0.7	>50	>5.8	345	Fed-batch	Reinhart et al. (2015)
	1.3	0.8	>2	0.04	2	Batch	Lee et al. (2005)
	27–33.5	n.a.	>2	0.09–0.015	58–72	Perfusion	Lee et al. (2005)
	>10	0.7	57	>1	85	Fed-batch	Zboray et al. (2015)
	25; 26	n.a.	35; 49	10; 13	530; 730	Fed-batch	Huang et al. (2010)
	n.a.	n.a.	n.a.	9.8	470	Fed-batch	Luan et al. (2006)
	>200	n.a.	10–15	n.a.	n.a.	Perfusion	Clincke et al. (2011)
	<110	n.a.	9–13	n.a.	n.a.	Perfusion	Clincke et al. (2013)
PER.C6	5	0.5	n.a.	0.3–0.5	42	Batch	Jones et al. (2003)
	>4	0.5	14–24	0.39	n.a.	Batch	De Kruif et al. (2010)
	n.a.	n.a.	n.a.	8	n.a.	Fed-batch	Kuczewski et al. (2011)
	≥150	n.a.	n.a.	27a	n.a.	Perfusiona	Kuczewski et al. (2011)
HEK 293b	n.a.	n.a.	n.a.	≤0.3	43	Batch	Hasegawa et al. (2014)
	>2	n.a.	20	0.2	20	Batch	Nallet et al. (2012)
	2–4	n.a.	5–10	0.12–0.14c	24–28	Batch	Jäger et al. (2013)
	6–8	n.a.	5–10	0.1–0.6c	<100	Fed-batch	Jäger et al. (2013)
NS0	0.6–1.0	0.3–0.6	20–50	0.1–0.2	13–17	Batch	Ho et al. (2012)
	2.3	0.6–1.0	8–20	0.07	14	Batch	Spens and Häggström (2007)
	2.3–9.9	1.0	8–20	0.1–0.8	19–67	Fed-batch	Spens and Häggström (2007)
	2.4	0.5	1.9–13.4	0.24	17	Fed-batch	Stansfield et al. (2007)

Values for the specific growth rate refer to the exponential growth phase

n .a. not available

^aProduct and cell retention in an extreme density bioreactor

^bTransient mAb expression

^cProduct was a scFv-Fc antibody fragment