Table 2.

Growth parameters and maximum cell densities of a batch process of P. pastoris and CHO cells.

Parameter	Pichia pastoris	CHO cells
Calculated cell massa [ng/cell]	0.02	2
Maximum cell concentration [cells/L]	1–2 × 10 13	0.5–1 × 1011
Maximum cell wet weight (WCW) [g/L]	200–400	100–200
Maximum specific growth rate [per h]	0.15	0.03

Values in italics are estimated (calculated) since they are usually not quantified for the system. The specific gravity of cells is simplified with 1

^aCell mass is based on the assumption of spherical cells with a diameter of 15–16 μm for CHO cells and 3–4 μm for P. pastoris cells