Figure 4.
Combined Deficiency in FADD and RIPK3 Prevents IEC Death, Paneth Cell Loss, and Colitis in NEMOIEC-KO Mice
(A and F) Representative images of colon and ileal sections from mice with the indicated genotypes.
(B) Immunoblot analysis of protein extracts from primary small intestinal IECs from mice with the indicated genotypes and with the indicated antibodies.
(C and G) Graphs depicting histopathological scores of colon and ileal sections from mice with the indicated genotypes (n = 5 or 6 mice per genotype).
(D and I) Graphs depicting the percentage of crypts with cl. casp. 3 stained cells on colon and ileal sections from littermates with the indicated genotypes (n = 5 or 6 mice per genotype).
(E, J, and K) Graphs depicting mRNA levels of the indicated genes in the colon and ileum of mice with the indicated genotypes (n = 9 or 10 mice per genotype).
(H) Graph depicting Paneth cell scores of ileal sections from mice with the indicated genotypes (n = 5 or 6 mice per genotype).
Scale bars represent 100 μm.