Figure 3.
Vax1flox/flox:GnRHcre males have a micropenis and are hypogonadal. A, Image of 6-week-old WT, cHET and cKO males. Scale bar, 0.5 cm. B, Testes of adult mice. Scale bar, 0.5 cm. C, H&E staining of testes from adult WT, cHET, and cKO males. Scale bar, 10 μm. WT and cHET males have a well defined penis (white arrow), preputial separation (WT, cHET), full size testes, seminal vesicles and normal spermatogenesis, whereas cKO have a micropenis (white arrow), delayed preputial separation, very small testes and seminal vesicles, as well as an absence of spermatogenesis. D, Basal circulating LH and FSH levels. One-way ANOVA compared with control. *p < 0.05. E, Circulating LH levels after GnRH and (F) kisspeptin-10 (kiss-10) challenges. Numbers over the histogram indicate fold-change after GnRH or Kiss-10 challenge; n = 4–8.