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. 2016 Mar 23;36(12):3506–3518. doi: 10.1523/JNEUROSCI.2723-15.2016

Figure 4.

Figure 4.

Vax1flox/flox:GnRHcre females have delayed pubertal onset and are hypogonadal. A, Pubertal onset was determined by vaginal opening (WT and cHET, white arrow). Panel shows images of 6-week-old females. Scale bar, 0.5 cm. B, Ovaries and uteri were collected from 25-week-old females. Scale bar, 0.5 cm. C, H&E-stained ovary. CL, Corpora lutea. Black arrows show stage 2 primordial follicles with the expected granulosa cell layer (black arrows), and some without (yellow arrows). Scale bar, 0.5 mm. D, Estrus cycling was monitored daily in adult 12- to 20-week-old WT, cHET, and cKO mice. M, Metestrus; E, estrus; P, proestrus; D, diestrus. E, Representative cell morphology for 5 d in WT and cKO females. F, Diestrus circulating LH and FSH levels. One-way ANOVA; **p < 0.01, ***p < 0.001. Circulating LH levels after (G) GnRH and (H) kisspeptin-10 (kiss-10) challenges. Numbers over the histograms indicate fold-change after GnRH or Kiss-10 challenge. n = 6–11.