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. 2016 Mar 23;36(12):3531–3540. doi: 10.1523/JNEUROSCI.3579-15.2016

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Copyright © 2016 the authors 0270-6474/16/363531-10$15.00/0

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Figure 7. — GLP-1-containing axons form close appositions with NTS astrocytes. Representative 1.5-μm-thick images from NTS-containing transverse sections (n = 3 rats) show GLP-1 in red, GFAP (astrocyte marker) in blue, and synaptophysin in green. White arrows indicate triple labeling, providing evidence for synaptic-like contacts between GLP-1-containing cells and astrocytes in the NTS. The top orthogonal in A represents the x–z plane for the z-stack, whereas the right orthogonal represents the y–z plane for the z-stack. The cross hairs in this panel (red vertical line meets green horizontal line) highlight one of several examples of three-label evidence for appositions at this level of the NTS. The inset in B reveals this at higher resolution, with the three-label example at the cross hairs in A represented by the top arrow in B. AP, Area postrema.