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. 2016 Mar 23;36(12):3541–3551. doi: 10.1523/JNEUROSCI.4124-15.2016

Figure 1.

Figure 1.

SNC80, but not ARM390, has increased potency and duration of action, and decreased acute tolerance in arrestin 2 KO mice. In a CFA model of inflammatory pain, we performed a dose and time response to SNC80 and ARM390. The anti-hyperalgesic effect of SNC80 (IP; A) was significantly enhanced in arrestin 2 KO mice (p < 0.05), and was longer lasting (B) (p < 0.001). C, D, ARM390 (PO) was equipotent in arrestin 2 WT and KO mice (C) and anti-hyperalgesic effects were lost 4 h after administration regardless of genotype (D). For acute tolerance experiments, Injection 1 represents mechanical responses in WT (E) and arrestin 2 KO (F) mice acutely treated with vehicle (control), SNC80 (3 mg/kg), or ARM390 (3 mg/kg). For Injection 2, animals were rechallenged with the same drugs and doses 4 h after Injection 1. Dashed lines represent baseline mechanical responses pre-CFA. For dose/time responses, two-way ANOVA was used (n = 5–7 mice/group). For acute tolerance, paired t tests with Bonferroni correction were used (***p < 0.001; n = 7–10 mice/group). SNC80 is more effective in arrestin 2 KO mice.