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. 2015 Dec 30;37(2):206–214. doi: 10.1093/carcin/bgv250

Figure 3.

Figure 3.

(A) Representative image of direct BE (apoptotic cell located one or two cells away from damaged cell) and indirect BE (apoptotic cells with no damaged cells in the field of interest) at 12h post-AOM injection. An apoptotic cell immediately adjacent to a damaged cell is defined as P1; an apoptotic cell located one cell away from a damaged cell is defined as P2 (right). Representative images (objective, 40×) of TUNEL+ (apoptotic body, red) cells next to γH2AX+ (DSBs, white) cells are shown counter-stained with DAPI (blue). (B) Percentage of BE-dependent and BE-independent apoptotic Lgr5+ stem versus differentiated cells (left) and percentage of BE-dependent apoptotic cells in relation to its proximity to a damaged cell.