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. 2016 Mar 22;7:10993. doi: 10.1038/ncomms10993

Figure 5. Smo influences expression of miR-378.

Figure 5

(a) qRT–PCR of the expression of Hh signals, including smo, gli2, gli3, gli1 and ptc, and profibrotic genes, including tgf-β, α-sma and col1α, in LX2 cells treated with (white bar) or without (black bar) 1 μM of GDC-0449, a Smoothened (Smo) antagonist, for 12, 24 and 48 h. (b) qRT–PCR analysis of miR-378a precursor (pri-miR-378a), miR-378b precursor (pri-miR-378b) and miR-378d precursor (pri-miR-378d) in LX2 cells treated with (white bar) or without (black bar) GDC-0449 for 12, 24 and 48 h. (c) qRT–PCR analysis of mature family members, including miR-378a-3p, miR-378b and miR-378d, in LX2 cells treated with (white bar) or without (black bar) GDC-0449 for 12, 24 and 48 h. All results of relative expression values are shown as mean±s.e.m. obtained from triplicate experiments (unpaired two-sample Student's t-test, *P<0.05 and **P<0.005 versus vehicle).

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