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. 2016 Mar 22;7:10993. doi: 10.1038/ncomms10993

Figure 9. Expression of miR-378 declines in injured liver of MCDE-fed mice and of patients with HCC.

Figure 9

(a) qRT–PCR of pro-fibrotic markers, vimentin, tgf-β, α-sma and col1α1, in mice fed with normal chow (CON) or a methionine/choline-deficient diet supplemented with 0.1% ethionine (MCDE) for 3 and 4 weeks (n=4 per group). Mean±s.e.m. results are graphed (unpaired two-sample Student's t-test, * P<0.05 and ** P<0.005 versus CON). (b) Expression of miR-378 family members, including miR-378a-3p, miR-378b and miR-378d, in MCDE-treated mice was examined (n=4 per group). Mean±s.e.m. results are graphed (unpaired two-sample Student's t-test, *P<0.05 versus CON). (c) qRT–PCR of miR-378a-3p and gli3 in paired non-tumour (NT) and tumour (T) regions of liver tissue from patients with human hepatocellular carcinoma (HCC) (n=18 patients). Individual values represented by the relative expression are shown by dots and their mean±s.e.m. results presented for each group (paired two-sample Student's t-test, *P<0.05 and **P<0.005 versus NT). (d) Spearman's rank correlation between miR-378a-3p expression and gli3 expression in NT and T of HCC (Spearman's rank correlation analysis; r, correlation coefficient).

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