Table 1. Primer sequences used for cloning.
Gene | Primer sequences (5′–3′) |
---|---|
gli2 wt 3′UTR_F | TTTTCTCGAGTCTCTGGCTCTTGTGGTGTG |
gli2 wt 3′UTR_R | TTGCGGCCGCCACCAAATTTACTGCCTGGA |
gli3 wt 3′UTR_F | TTTTCTCGAGGAGTCAAAAGTGTTCTATCCCAAGA |
gli3 wt 3′UTR_R | TTGCGGCCGCTTCCTGGTGACACTGTCTGG |
gli2 mut 3′UTR_F | GTTTGGCAATATAAATTTGGGTCGTCCAAATTTGGATTCACCCACAGTC |
gli2 mut 3′UTR_R | GACTGTGGGTGAATCCAAATTTGGACGACCCAAATTTATATTGCCAAAC |
gli3 mut 3′UTR-1_F | CACCAATCTGTCCTGATTGACGACTTCAAGAATGATCATTTGG |
gli3 mut 3′UTR-1_R | CCAAATGATCATTCTTGAAGTCGTCAATCAGGACAGATTGGTG |
gli3 mut 3′UTR-2_F | CAGCCTCTGAGTGCTGACTCCTCAACAACAACAACTGGTCC |
gli3 mut 3′UTR-2_R | GGACCAGTTGTTGTTGTTGAGGAGTCAGCACTCAGAGGCTG |
+p65 BS_F | TTTGCTAGCCACTTGCTGCCGTACTTTCA |
+p65 BS_R | TTTTCTCGAGCTCCCACTCCAGGTACCAAA |
Δp65 BS_F | TTTGCTAGCGACAGCGTCTTCCTGGTTTC |
F, forward; mut, mutant; R, reverse; wt, wild-type.
Primer sequences shown in this table were used for cloning the vector constructs.