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. 2016 Mar 15;7:755–759. doi: 10.1016/j.dib.2016.03.038

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© 2016 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 2 — Knockdown of BCKDK gene in MSUD fibroblasts. Analysis of BCKDHc activity and bioenergetics profile. (A) BCKDHc activity was measured in MSUD fibroblast׳s extracts (MSUD); or after lentiviral-transduction with non-target control shRNA (sc) or shRNA1 (#1). Histogram represents the folds increases over basal. (B) Oxygen Consumption Rate -OCR- was measured as an evaluation of mitochondrial respiratory profile changes in control cells (C), non-transduced (MSUD) or lentiviral-transduced MSUD fibroblasts with non-target control (sc) or shRNA1 (sh1). Results are the mean±SD of 3–5 wells from n=2 independent experiments.