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. 2016 Mar 23;6:23591. doi: 10.1038/srep23591

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(a) Control experiment based on Calcein AM stained HL-60 cells shows that there is no remarkable change in the fluorescence intensity during the evaporation process. (b) The small enhancement factor obtained in the control experiment suggests that the sudden increase in the calcium response in our experiment is not caused by the dye enrichment itself.