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. 2016 Mar 23;6:23596. doi: 10.1038/srep23596

Figure 4. NFκB-p65 nuclear translocation involved in ACAT-1 and VCAM-1 transcriptions response to salusin-β in VSMCs.

Figure 4

(A) effects of different doses of salusin-β (1, 3, 10 or 30 nM) on NFκB-p65 nuclear translocation. (B) ChIP analysis was performed with the indicated antibodies, and qPCR was underwent with a synthesized DNA fragment containing the NFκB element in human ACAT-1 gene promoter or VCAM-1 promoter. (C) effects of a NFκB inhibitor Bay11-7082 on salusin-β-induced ACAT-1 and VCAM-1 expressions. (D) effects of Bay11-7082 on salusin-β-induced foam formation. (E) effects of Bay11-7082 on monocyte adhesion response to salusin-β. (F) effects of Bay11-7082 on intracellular cholesterol content and monocyte adhesion. VSMCs were incubated with Bay11-7082 for 6 h and then treated with or without salusin-β (30 nM) for 48 h. Values are mean ± S.E.M. *P < 0.05 and **P < 0.01 vs. 0 nM, Vehicle or PBS. †P < 0.05 and ††P < 0.01 vs. PBS+Salusin-β or Vehicle+Salusin-β; n = 4 for each group.