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. 2016 Mar 23;6:23593. doi: 10.1038/srep23593

Figure 1. α-GalCer expands porcine NKT-cells that are mostly CD4−.

Figure 1

(a) Representative flow cytometry plots comparing peripheral blood (PB) NKT-cells from a mock-vaccinated pig versus a pig vaccinated with α-GalCer. Single cell suspensions were membrane-labeled with anti-CD3 mAb and PBS57-loaded CD1d tetramer. Gating to discriminate CD4+ and CD4− NKT-cell subsets was based on CD4 staining after gating on CD3+ lymphocytes. (b) Frequency of NKT-cells as a percentage of CD3+ lymphocytes in PB of individual pigs during the vaccination period and post infection (shaded region). (c) Percentage of CD4− NKT-cells in PB during the vaccination period and post infection (shaded region). (d) Frequency of NKT-cells as a percentage of total lymphocytes in bronchoalveolar lavage fluid (BALF), lung, tracheobronchial lymph node (TBLN) and spleen at 3 and 7 days p.i. Differences in PB NKT-cell frequencies and subsets were analyzed using the SAS PROC MIXED procedure and the Turkey’s test was used to examine treatment differences at each time point for each dependent variable. Changes in BALF, lung, TBLN and spleen NKT-cell frequencies were analyzed using the Kruskal-Wallis test. *P < 0.05. Data are represented as mean ± SEM. Arrows indicate when vaccinations were administered.