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. 2016 Mar 23;6:22789. doi: 10.1038/srep22789

Figure 6. Evaluation of the energy status of human keratinocytes in vitro.

Figure 6

(a) Fluorescence intensity images reflecting the total amount of cellular NADH (first row) and fluorescence lifetime images (FLIM) (second row) of non-treated keratinocytes (control) or keratinocytes treated either with 20 mM glucose, 5 μM rotenone or 10 nM TNF-alpha. The colour-coded bar reflects the fluorescence lifetime, ranging from 1,000 ps (red) to 2,000 ps (blue). (b) Quantification of the mean fluorescence intensity of non-treated keratinocytes (co) or keratinocytes treated with glucose (glu), rotenone (rot), or TNF-alpha (TNF). (c) Quantification of the mean fluorescence lifetime (taum) of non-treated keratinocytes (co) or keratinocytes treated with glucose (glu), rotenone (rot), or TNF-alpha (TNF). *P < 0.05, **P < 0.01.