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. 2016 Mar 23;6:23496. doi: 10.1038/srep23496

Figure 2. Retina pigmentation in lamprey and zebrafish developed from Cas9 and gol gRNA injected embryos.

Figure 2

(a–f) Lateral views of control (a,d) and gol gRNA injected larvae (b,c,e,f) on 19 dpf (days post fertilization). Phenotypes shown include the wild-type (arrow in (d)), mosaic hypopigmentation (arrow in (e)) and no observable pigment (arrow in (f)) in the retina. Zebrafish embryos injected with Cas9 and the appropriate gol gRNA on 2 dpf also showed mosaic pigmentation (h,i) in the retinas. Scale bars: 0.2 mm.