Table 3. Larval heart malformations from Cas9 and kctd10 gRNA treatment.

Treatment	Total Number of larvae	Normal larvae	Larvae with heart disorders	Mutant (%)
Control	114	111	3	2.6
LCas9-1 + kctd10 gRNA	116	17	99	85.3
LCas9-2 + kctd10 gRNA	44	20	24	54.5
ZCas9 + kctd10 gRNA	54	16	38	70.4

Overall, treatment of Cas9 affected the phenotype (χ² = 169.61, p < 0.0001). Treatment by each one of the three Cas9s induced more malformations than the vehicle treatment (p < 0.0001 for each comparison). LCas9-1 induced a higher rate of malformation in the heart than LCas9-2 (χ² = 17.02, p < 0.0001) and ZCas9 (χ² = 5.28, p = 0.022). LCas9-2 did not differ from ZCas9 in the number of induced malformations (χ² = 2.613, p = 0.10).