Table 1.

Significant difference among 24, 48, and 72 h after treatment (repeated‐measures ANOVA)

Cell lines	Concentration (nmol/L)	Type III sum of squares	df	Mean square	F value	P value
MCF‐7	10	0.026	2	0.013	4.755	0.035
	100	0.279	2	0.140	29.314	0.000
	200	0.338	2	0.169	56.770	0.000
	500	0.546	2	0.273	76.405	0.000
MDA‐MB‐231	10	0.033	2	0.016	2.401	0.141
	100	0.078	2	0.039	4.566	0.039
	200	0.244	2	0.122	15.192	0.001
	500	0.629	2	0.314	31.033	0.000
Hs 578Bst	10	0.046	2	0.023	0.993	0.404
	100	0.113	2	0.056	2.728	0.113
	200	0.070	2	0.035	2.488	0.133
	500	0.037	2	0.019	0.947	0.420

Based on MTT analysis, MCF‐7, MDA‐MB‐231 and Hs 578Bst were exposed to deguelin at indicated concentrations (0–500 nmol/L) for 24, 48, 72 h. Cell viability was measured by a spectrophotometer. A repeated‐measures ANOVA was used to see if the time points are significantly different to each other in three cell lines. As the P values in Mauchly's Test of Sphericity are all more than 0.05, sphericity has not been violated. The results in “sphericity assumed” in SPSS were presented in the table. ANOVA, analysis of variance; MTT, 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide.

*P < 0.05; **P < 0.01; ***P < 0.001.