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. Author manuscript; available in PMC: 2016 Mar 23.
Published in final edited form as: Biochem J. 2011 Apr 1;435(1):259–266. doi: 10.1042/BJ20102003

Figure 3. A candidate ZRE in the ZRG17 promoter is required for its induction.

Figure 3

A) β-galactosidase activity was measured in wild-type cells (DY1457) or zap1Δ mutant cells (ZHY6) bearing the wild-type ZRG17-lacZ reporter and grown in LZM over a range of zinc concentrations. B) The ZRG17 promoter with the wild-type ZRE (ZRG17 ZRE) and two different promoter mutants constructed (m1ZRE, m2ZRE). Both ZRG17 m1ZRE and ZRG17 m2ZRE were mutated such that each of the eleven positions of the ZRE was altered by transversion mutations. ZRG17 m2ZRE has three additional base pairs (TGA) adjacent to the ZRE deleted. C) β-galactosidase activity was measured in wild-type cells (DY1457) bearing the vector (YEp353), the wild-type ZRG17-lacZ reporter (WT), or the mutated reporters (ZRG17-m1ZRE, ZRG17-m2ZRE) and grown in low zinc (LZM + 1 μM ZnCl2) or high zinc (LZM + 1000 μM ZnCl2) conditions. The data shown are the means of three independent cultures for each condition and are representative of two independent experiments. The error bars represent ± 1 S.D.