Table 1.
ZRE | Oligonucleotide |
---|---|
TSA1 ZRE1 | 5′-ggccCTGTTCTGGCCCGTCGGGTTTTCTGACAAA-3′ |
3′-GACAAGACCGGGCAGCCCAAAAGACTGTTTagct-5′ | |
ZRG17 ZRE | 5′-ggccACTGAAAATGATGAACCTTGAAGGTATTTTTGTTACT-3′ |
3′-TGACTTTTACTACTTGGAACTTCCATAAAAACAATGAagct-5′ | |
ZRG17 m1ZRE | 5′-ggccACTGAAAATGATGACAAGGTCCTTGATTTTTGTTACT-3′ |
3′-TGACTTTTACTACTGTTCCAGGAACTAAAAACAATGAagct-5′ |
ZRG17 m1ZRE was mutated such that each position in the potential ZRE was altered by a transversion mutation. ZREs or regions mutated in each complementary oligonucleotide pair are indicated by the underline. The lower-case letters indicate EagI- and SalI-complementary overhangs included for cloning these fragments into a lacZ reporter plasmid for future studies.