Skip to main content
. Author manuscript; available in PMC: 2016 Mar 23.
Published in final edited form as: Biochem J. 2011 Apr 1;435(1):259–266. doi: 10.1042/BJ20102003

Table 1.

Oligonucleotides used for EMSAs

ZRE Oligonucleotide
TSA1 ZRE1 5′-ggccCTGTTCTGGCCCGTCGGGTTTTCTGACAAA-3′
  3′-GACAAGACCGGGCAGCCCAAAAGACTGTTTagct-5′
ZRG17 ZRE 5′-ggccACTGAAAATGATGAACCTTGAAGGTATTTTTGTTACT-3′
  3′-TGACTTTTACTACTTGGAACTTCCATAAAAACAATGAagct-5′
ZRG17 m1ZRE 5′-ggccACTGAAAATGATGACAAGGTCCTTGATTTTTGTTACT-3′
  3′-TGACTTTTACTACTGTTCCAGGAACTAAAAACAATGAagct-5′

ZRG17 m1ZRE was mutated such that each position in the potential ZRE was altered by a transversion mutation. ZREs or regions mutated in each complementary oligonucleotide pair are indicated by the underline. The lower-case letters indicate EagI- and SalI-complementary overhangs included for cloning these fragments into a lacZ reporter plasmid for future studies.